Dwi Nugroho Juanda

Novel Therapeutic Strategies for Peri-implantitis with Hypoxia-Induced Mesenchymal Stem Cells (MSCs) Introduction: Periimplantitis, which entails chronic inflammation within the peri-implant tissue, ultimately leading to aggressive bone resorption. Recently, conventional treatment approaches frequently struggle to effectively treat periimplantitis and promote bone regeneration. Stem cell-based therapy using Mesenchymal Stem Cells (MSCs) has emerged as a promising candidate for regenerative treatment. Hypoxia-induced MSCs, as a potential strategy due to their profound impact on the generation of growth factors, can enhance their regenerative and immunomodulatory capabilities. Objective: This study aimed to evaluate the effect of hypoxia-induced MSCs to promote bone regeneration on peri-implantitis models. Methods: 42 Wistar strain Rattus norvegicus divided into 6 groups: peri-implantitis group 14 days (K1), 28 days (K2); periimplantitis + normoxia MSCs group 14 days (PN1), 28 days (PN2); periimplantitis + hypoxia MSCs group 14 days (PH1), 28 days (PH2). The expression levels of TNF-α, NFATc1, as an inflammation marker, and COL1α1, osteocalcin, as a bone regeneration marker, were examined and statistically analyzed using one-way ANOVA. Results: Hypoxia-induced MSCs significantly reduce TNF-α and NFATc1 and elevate COL1α1 and osteocalcin expression. Conclusion: The transplantation of hypoxia-induced MSCs promotes a capacity to ameliorate inflammation and enhance bone regenerative processes in periimplantitis models. Keywords: Dental Implant, Mesenchymal Stem Cell, Peri-implantitis, Stem cell-based therapy

Dwi Nugroho Juanda graduated from Trisakti University, Jakarta in 2018, and is currently pursuing a senior year in the prosthodontic specialist program at Airlangga University, Surabaya. His notable contributions to the field of implants and prosthodontics include publications such as “Alendronate Effect in RANKL Concentration at Ovariectomized Sprague Dawley Rat” (2018) and “The Effect of Alendronate to Osseointegration of Dental Implant at Ovariectomized Sprague Dawley Rat” (2019). Dwi’s achievements include finalist status at the APDSA Taiwan in 2015, second place at the IADR South East Asian Division in 2015, and second place at the Best Case Report Competition in the “1st Indonesia Prosthodontic Society Meeting & IPROSI Congress XI” in 2022. Additionally, Dwi has demonstrated a commitment to dental education through his private dental anatomy workshop, providing courses for dentists and dental technicians for over 5 years.